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Biotechnology Reports Biochemical Findings From Phase IIa Study With Hypertension Vaccine CYT006-AngQb

Cytos Biotechnology Ltd (SIX:CYTN) announced results from a biochemical analysis from a phase IIa study (study 02) with the vaccine candidate CYT006-AngQb for the treatment of hypertension. CYT006-AngQb has demonstrated in a first phase IIa study (study 01) a significant reduction of the day-time ambulatory blood pressure of -9 / -4 mmHg (systolic/diastolic) vs. placebo (The Lancet 2008, 371:821). In study 02 an accelerated treatment regimen with injections at weeks 0, 2, 4, 6, and 10 was tested, while in study 01 the vaccine was given at weeks 0, 4, and 12. This modification was anticipated to induce higher antibody titers and, thereby, a stronger blood pressure reduction. While first study results which were communicated on March 17, 2009 showed on average a 5-fold higher antibody titer in study 02 than in study 01, the blood pressure reductions in study 02 were much lower than in study 01; they amounted to -2.3 / -0.4 mmHg. In order to understand this discrepancy, the biochemical properties of the induced antibody responses were analyzed in detail. The main findings are as follows: - Antibody affinities (i.e. the strength by which the antibodies bind angiotensin II) determined by different biochemical methods were significantly lower in study 02 than in study 01 (pAbout the hypertension vaccine CYT006-AngQb CYT006-AngQb is a therapeutic vaccine in development for the treatment of hypertension1,2. It is designed to instruct the patient"s immune system to produce an antibody response against angiotensin II. Angiotensin II is a small peptide in the body and part of the renin-angiotensin system (RAS), which is an important regulator of blood pressure. Angiotensin II causes blood vessels to narrow, resulting in increased blood pressure. In a phase IIa study with hypertensive patients, vaccination with CYT006-AngQb has been shown to significantly reduce the mean ambulatory daytime blood pressure by induction of antibodies that bind angiotensin II (The Lancet 2008, 371:821). A particularly strong blood pressure reduction has been observed in the early morning hours - a crucial time of day when adverse cardiovascular events are more likely to occur than during other times of the day. CYT006-AngQb is a first-in-class product candidate in this important indication and represents a completely novel approach to hypertension treatment. Treatment with CYT006-AngQb should allow for convenient dosing schedules and a smooth control of blood pressure due to a sustained antibody response induced by vaccination. About hypertension Hypertension, also termed high blood pressure, is a medical condition where the blood pressure is chronically elevated. Although symptomless in nature and in itself rarely an acute problem, persistent hypertension is one of the most important preventable causes of premature death worldwide and contributes to around half of all cardiovascular diseases3. It is one of the major risk factors for stroke, myocardial infarction, heart failure, and vascular disease, and is a leading cause of chronic renal failure. Genetic predisposition and lifestyle habits such as inadequate physical activity, high fat diet, and high salt intake promote high blood pressure. Up to 30% of adults in most countries suffer from hypertension. Despite effective and relatively inexpensive treatment available, less than one out of four hypertensive individuals have their blood pressure controlled successfully4,5. This poor overall treatment success is mainly attributed to the symptomless nature of hypertension and the necessity for long-term treatment with currently available medications that require at least once daily self-administration. References 1. Journal of Hypertension; A vaccine for hypertension based on virus-like particles: preclinical efficacy and phase I safety and immunogenicity; 2007, 25:63 2. The Lancet; Effect of immunization against angiotensin II with CYT006-AngQb on ambulatory blood pressure: a double-blind, randomized, placebo-controlled phase IIa study; 2008, 371:821 3. Centres for Disease Control and Prevention (CDC); The Atlas of Heart Disease and Stroke, 2004 4. Journal of the American Medical Association (JAMA); The Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure; 2003, 289:2560 5. National Institute for Health and Clinical Excellence (NICE), Centre for Health Services Research, UK; Essential Hypertension: managing adult patients in primary care; August 2004 Cytos Biotechnology Ltd


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